WHO workshop and practical course on Human Papillomavirus (HPV) genotyping and HPV16/18 serology

Overview

The first meeting of the global HPV laboratory network (LabNet) was organized as a joint workshop and practical course. The aims of the workshop were to discuss laboratory aspects linked to the introduction, follow-up and surveillance of the HPV prophylactic vaccines and the role of the global HPV LabNet in promoting internationally recognized quality of the laboratory functions needed. The practical course allowed the participants to provide input on both theoretical and practical issues involved in international standardization and quality control of HPV laboratory methodologies. The course provided an opportunity for participants to conduct HPV genotyping on patient samples and a candidate HPV LabNet DNA proficiency panel using consensus primer PCR and reverse hybridization methodology. Participants also performed HPV serology on patient samples and a candidate International Standard (IS) for HPV16 antibody using both a virus-like particle (VLP)-based enzyme-linked immunosorbent assay (ELISA) and a pseudovirion (PsV)-based neutralization assay. The workshop reviewed the tasks of the appointed HPV LabNet members and made a number of practical decision regarding organization and implementation of the specific tasks of the HPV LabNet, such as the organization of the writing of the WHO Laboratory Manual for HPV diagnosis and the design of the HPV LabNet communication strategy including a bi-annual Newsletter. The workshop/practical course was positively evaluated by the participants and can be used as a template for forthcoming workshops and practical courses for newly appointed HPV LabNet members or trainees involved in HPV surveillance.